Histone H2B anticorps (meLys5)

N° du produit ABIN6971819

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone H2B dans WB et DB. Il présente une réactivité envers Humain.

Aperçu rapide pour Histone H2B anticorps (meLys5) (ABIN6971819)

Antigène: Histone H2B

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2B est non-conjugé

Application: Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-Histone H2B anticorps

Épitope: meLys5

Attributs du produit: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Changes in methylation of histone H2B may be involved in heat-shock mechanisms. Monomethylation of Lysine 5 has been reported to occur at active promoters downstream of the TSS. Histone H2BK5me1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogène: This antibody was raised against a synthetic peptide containing monomethyl Lysine 5 of human histone H2B.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Histone H2B

Antigène: Histone H2B

Poids moléculaire: 15 kDa

NCBI Accession: NP_003512